Impact of granulocyte colony stimulating factor infusion on the implantation rate in women with unexplained previous intracytoplasmic sperm injection failure: a case control study


Objective. We studied the granulocyte colony stimulating factor (G-CSF)’s effect on women with unexplained infertility and previous intracytoplasmic sperm injection (ICSI) failure. Unexplained infertility is a condition when couples are not able to conceive despite normal semen analysis and absence of female infertility factor(s). Cytokines, transcription factors and signaling pathways are essential for complex interactions of decidualization. G-CSF is a glycoprotein with growth factor and cytokine functions, which are produced in many tissues.
Patients and Methods. The study was done on a total of 200 women with only 196 women who ended the study (99 women as a study group, 96 women as a control group). All women were complaining of unexplained infertility with previous history of failed ICSI. All cases proceed through ICSI procedure while study group only undergone G-CSF infusion intrauterine at time of ovum pickup.
Results. We found that intrauterine G-CSF injection at time of ovum pickup in the study group, in comparison with control group, did not improve neither implantation rate (16.68% vs 19.66%, p = 0.243) nor the chemical (54.5% vs 67%, p = 0.074), clinical pregnancy (51.5% vs 62.9%, p = 0.108) rates as well as live birth rates (31.0% vs 39.8%, p = 0.227).
Conclusions. Intrauterine infusion of G-CSF may not improve implantation rate in women with unexplained previous intracytoplasmic sperm injection (ICSI) failure. Further studies are needed to conclude if the dose, route and timing of the drug administration can give better results.

Table of Content: Vol. 36 (No. 2) 2024 June

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